ÎÒÄÅË ÔÓÍÊÖÈÎÍÀËÜÍÎÉ ÁÈÎÕÈÌÈÈ ÁÈÎÏÎËÈÌÅÐÎÂ
ÂÑÅÂÎËÎÄ ÀÐÑÅÍÜÅÂÈ× ÒÊÀ×ÓÊ
çàâåäóþùèé îòäåëîì ñ 1998 ã., ÷ëåí-êîððåñïîíäåíò ÐÀÍ, ÷ëåí-êîððåñïîíäåíò ÐÀÌÍ, äîêòîð áèîëîãè÷åñêèõ íàóê, ïðîôåññîð, çàâ. êàôåäðîé ìåäèöèíñêîé áèîõèìèè ôàêóëüòåòà ôóíäàìåíòàëüíîé ìåäèöèíû ÌÃÓ
Ñðàâíåíèå ïîëèìåðèçàöèè èíòàêòíîãî è ìîäèôèöèðîâàííîãî ïî òèðîçèíó ôëàãåëëèíà ïîäòâåðäèëî, ÷òî ñóáúåäèíèöà ôëàãåëëèíà âêëþ÷àåòñÿ â ïîëèìåð, òîëüêî ïðèíÿâ îïðåäåëåííóþ êîíôîðìàöèþ, ïîçâî-ëÿþùóþ åé âñòðîèòüñÿ â ïîëèìåð.
Áîëåå 20 ëåò ïðîâîäÿòñÿ èññëåäîâàíèÿ ñòðóêòóðû è ôóíêöèé ãëàâíûõ áåëêîâ ñîêðàòèòåëüíîãî àïïàðàòà ìûøö è ìíîãèõ èíûõ äâèãàòåëüíûõ ñèñòåì - ìèîçèíà è àêòèíà.  òå÷åíèå ïîñëåäíèõ 8 ëåò ïðîâîäÿòñÿ ñèñòåìàòè÷åñêèå èññëåäîâàíèÿ ñòðóêòóðû ìèîçèíîâîé ãîëîâêè è àêòèíà ìåòîäîì äèôôåðåíöèàëüíîé ñêàíèðóþùåé êàëîðèìåòðèè.
 ëàáîðàòîðèè áåëêîâ ðàñòåíèé (ä.á.í. Ì.À.Áåëîçåðñêèé) èçó÷åíà ðåãóëÿòîðíàÿ ñèñòåìà ïðîòåîëèçà çàïàñíûõ áåëêîâ â âûñøèõ ðàñòåíèé. Âåäóòñÿ ðàáîòû ïî èññëåäîâàíèþ èíãèáèòîðîâ ïðîòåàç êàê ñîñòàâíîé ÷àñòè çàùèòíîé ñèñòåìû ðàñòåíèé îò ïàòîãåíîâ. Ðàçðàáàòûâàþòñÿ ýêîëîãè÷åñêè ÷èñòûå ìåòîäû óñòîé÷èâîñòè ðàñòåíèé ê ïàòîãåíàì.
Èç êóëüòèâèðóåìûõ êëåòîê êèòàéñêîãî õîìÿ÷êà ÑÍÎ êëîíèðîâàí ôðàãìåíò (629 àìèíîêèñëîòíûõ îñòàòêîâ) íîâîãî áåëêà p3D2 ñ ìîëåêóëÿðíîé ìàññîé 210 êÄà, èìåþùåãî áîëåå 70% ãîìîëîãèè ñ ïðîòåèíêèíàçàìè ÷åëîâåêà è êðûñû.  êóëüòèâèðóåìûõ êëåòêàõ ìëåêîïèòàþùèõ p3D2 ëîêàëèçîâàí â èíòåðõðîìàòèíîâûõ ðàéîíàõ ÿäðà, öåíòðîñîìå è àññîöèèðîâàí ñ ìèêðîòðóáî÷êàìè. Ïðåäïîëîæèòåëüíî, p3D2 ó÷àñòâóåò â ðåãóëÿöèè äèíàìèêè ìèêðîòðóáî÷åê è/èëè çàâèñèìûõ îò ìèêðîòðóáî÷åê ïðîöåññîâ.
Èçáðàííûå ðàáîòû
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2. Novikov V.V., Metlina A.L., Poglazov B.F. A study on the mechanism of polymerisation of Bacillus brevis flagellin. Biochem. Mol. Biol. Internat. (1994) 33, 723-728.
3. Levitsky D.I., Shnyrov V.L., Khvorov N.V., Bukatina A.F., Vedenkina N.S., Permykov E.A., Nikolaeva O.P. Poglazov B.F. Effects of nucleotide binding on thermal transitions and domain structure of myosin subfragment 1. Eur. J. Biochem. (1992) 209, 829-835.
4. Zinovkina L.A., Poltaraus A.B.., Solovyanikova O.B., Nadezhdina E.S. Chinese hamster protein homologous to human putative protein kinase KIAA0204 is associated with nuclei, miceotubules and centrosomes in CHO-K1 cells. FEBS Lett. (1997) 414, 135-139.
5. Belozersky M.A., Dunaevsky Y.E., Mussolyamov A.X., Egorov T.A. Complete amino acid sequence of the protease inhibitor from buchwheat seeds. FEBS Lett. (1995) 371, 264-266.