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Main results: Cancer serology. Serological screening of expression cDNA libraries from
renal, breast, ovarian and colon cancers and from cancer cell lines and
normal testis (approximately 2000000 clones tested, out of which 443
serologically positive clones were submitted to Cancer Immunome Database,
SEREX project). Three novel human cancer antigens discovered, and seven
previously described proteins shown to be putative cancer antigens [Lagarkova
et al., 2000; Lagarkova et al., 2003; Kuimov et al., 2001; Shebzukhov et al.,
2005a, Shebzukhov et al., 2005b]. We developed an improved method to detect
serological responses to tumor-associated antigens using a panel of
cancer-specific antigens for serologic diagnostics and monitoring of
melanoma, renal cancer, breast cancer, ovary cancer, lung cancer and colon
cancer. It allowed simultaneous detection of responses to multiple antigens.
New version of the method utilizes recombinant proteins purified by
chromatography. A library of clinical specimens (blood serum samples and
tissue specimens from patients with different cancers) characterized by panel
of cancer-specific antigens was collected. Physiological functions of TNF and LT. A unique panel of
mice with complete and tissue-specific inactivation of tumor necrosis factor
(TNF) and lymphotoxin (LT) genes was generated. Our data revealed distinct
functions for LT produced by B-lymphocytes [Tumanov et al., 2002] and for TNF
produced by macrophages and T-cells [Grivennikov et al., 2005], and demonstrated
cooperation of TNF and LT in vivo
[Kuprash et al., 2002]. Molecular mechanism of the action of cyclosporin A on
expression of LTα gene was established [Kuprash et al., 2002]. Mouse
monoclonal antibodies against genetically cloned TNF were sequenced [Radko et
al., Russ. J. Immunol. 7: 371, 2002] and used for generation of bioengineered
TNF inhibitor. A novel animal model for studing the consequences of TNF
blockade in vivo has been
generated: mice with “humanized” TNF/LT locus. |
Last
update April 16, 2007 by Dmitry
Kuprash and Maria Goncharskaya